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Metal Homeostasis in the Plant-Microorganism Interaction

Manuel González-Guerrero (IP)

Assistant Professor
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigaciones Agrarias, Madrid

0000-0001-7334-5286

manuel.gonzalez@upm.es

PHONE NUMBER

+ 34 910679190 Ext:79190

Other group members

Emma Barahona Martín

Postdoc

Viviana P. Escudero Welsch

Postdoc

Cristina Navarro Gómez

Predoc

Elena Rosa Núñez

Predoc

Sara Sopeña Torres

Technician

Research lines

  • Transport of metals in legume nodules
  • Metal exchange in arbuscular mycorrhizae
  • Metal trafficking in biological nitrogen fixation
  • Transition metals and innate plant immunity

Representative publications

  • Senovilla M, Abreu I, Escudero V, Cano C, Bago A, Imperial J, González-Guerrero M (2020) MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots. Mycorrhiza 30: 781-788.
  • Castro-Rodríguez R, Abreu I, Reguera M, Novoa-Aponte L, Mijovilovich A, Escudero V, Jiménez-Pastor FJ, Abadía J, Wen J, Mysore KS, Álvarez-Fernández A, Küpper H, Imperial J, González-Guerrero M (2020) Medicago truncatula Yellow Stripe1-Like3 gene is involved in symbiotic nitrogen fixation. J Exp Bot (doi: 10.1093/jxb/eraa390).
  • Escudero V, Abreu I, Tejada-Jiménez M, Rosa-Núñez E, Quintana J, Prieto RI, Larue C, Wen J, Villanova J, Mysore KS, Argüello JM, Castillo-Michel H, Imperial J, González-Guerrero M (2020) Medicago truncatula Ferroportin2 mediates iron import into nodule symbiosomes. New Phytol 228: 194-209.
  • Escudero V, Abreu I, del Sastre E, Tejada-Jiménez M, Larue C, Novoa-Aponte L, Castillo-González J, Wen J, Mysore KS, Abadía J, Argüello JM, Castillo-Michel H, Álvarez-Fernández A, Imperial J, González-Guerrero M (2020) Nicotianamine synthase2 is required for symbiotic nitrogen fixation in Medicago truncatula Front Plant Sci 10: 1780.
  • Ibeas MA, Grant-Grant S, Coronas MF, Vargas JI, Navarro N, Abreu I, Castillo-Michel H, Ávalos-Cembrano N, Paez-Valencia J, Pérez F, González-Guerrero M, Roschzttardtz H (2019) The diverse iron distribution in Eudicotyledoneae seeds: from Arabidopsis to Quinoa. Front Plant Sci 9: 1985 (doi: 3389/fpls.2018.01985).
  • Gil-Díez P, Tejada-Jiménez M, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2019) MtMOT1.2 is responsible for molybdate supply to Medicago truncatula Plant Cell Environ 42: 310-320.
  • León-Mediavilla J, Senovilla M, Montiel J, Gil-Díez P, Saez A, Kryvoruchko IS, Reguera M, Udvardi MK, Imperial J, González-Guerrero M (2018) MtMTP2-facilitated zinc transport into intracelular compartments is essential for nodule development in Medicago truncatula. Front Plant Sci 9: 990 (doi: 10.3389/fpls.2018.00990).
  • Senovilla M, Castro-Rodríguez R, Abreu I, Escudero V, Kryvoruchko I, Udvardi MK, Imperial J, González-Guerrero M (2018) Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation. New Phytol 218: 696-709.
  • Kryvoruchko I, Routray P, Senjuti S, Torres-Jerez I, Tejada-Jiménez M, Finney LA, Nakashima J, Pislariu CI, Benedito VA, González-Guerrero M, Roberts DM, Udvardi MK (2018) An iron-activated citrate transporter, MtMATE67, is required for symbiotic nitrogen fixation. Plant Physiol 176: 2315-2329.
  • Tejada-Jiménez M, Gil-Díez P, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2017) Medicago truncatula Molybdate Transporter type1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency. New Phytol 216: 1223-1235.
  • Abreu I, Sáez A, Castro-Rodríguez R, Escudero V, Rodríguez-Haas B, Senovilla M, Larue C, Grolimund D, Tejada-Jiménez M, Imperial J, González-Guerrero M (2017) Medicago truncatula Zinc-Iron Permease6 provided zinc to rhizobia-infected nodule cells. Plant Cell Environ 40: 2706-2719.
  • González-Guerrero M, Escudero V, Saéz A, Tejada-Jiménez M (2016) Transition metal transport in plants and associated endosymbionts: Arbuscular mycorrhizal fungi and rhizobia. Front Plant Sci 29:1088 (doi: 10.3389/fpls.2016.01088).
  • Tejada-Jiménez M, Castro-Rodríguez R, Kryvoruchko IS, Lucas MM, Udvardi M, Imperial J, González-Guerrero M (2015) Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia-infected nodule cells. Plant Physiol 168: 258-275.
  • Zielazinski EL*, González-Guerrero M*, Subramanian P, Stemmler TL, Argüello JM, Rosenzweig AC (2013) Sinorhizobium meliloti Nia is a P1B-5-ATPase expressed in the nodule during plant symbiosis and is involved in Ni and Fe transport. Metallomics 5: 1614-1623.
  • Rodríguez-Haas B, Finney L, Vogt S, González-Melendi P, Imperial J, González-Guerrero M (2013) Iron distribution through the developmental stages of Medicago truncatula Metallomics 5: 1247-1253.

Grants

  • Rubio L (PI), González-Guerrero M (2020-2024) BNF-Cereals Phase III. Iron and Mo delivery to pathways to nitrogenase. Bill & Melinda Gates Foundation.
  • González-Guerrero M (PI) (2019-2021) Role of plant Cu+-chaperones in intracellular copper trafficking in symbiotic nitrogen fixation. Ministry of Science, Innovation and Universities. PGC2018-095996-B.
  • González-Guerrero M (PI) (2016-2018) Diverting metals to Medicago truncatula Ministry of Economy and Competitiveness. AGL-2015-65866-P.
  • González-Guerrero M (PI) (2014-2019) Metal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia. European Research Council Starting Grant.

Relevant methods

  • Confocal and electronic microscopy
  • Visualisation of transition metals in plant tissues
  • Purification of membrane proteins
  • Metal transport tests on yeasts

Collaborations with other national and international groups

    • Luis M. Rubio (Centro de Biotecnología y Genómica de Plantas, UPM-INIA)
    • Lucía Jordá (Centro de Biotecnología y Genómica de Plantas, UPM-INIA)
    • Juan Imperial (Instituto de Ciencias Agrarias, CSIC)
    • Alberto Bago (Estación Experimental del Zaidín, CSIC)
    • Javier Abadía, Ana Álvarez (Estación Experimental de Aula Dei, CSIC)
    • José M. Argüello (Worcester Polytechnic Institute, EEUU)
    • Luis Vidali (Worcester Polytechnic Institute, EEUU)
    • Dennis Dean (Virginia Tech, EEUU)
    • Lydia Finney (Argonne National Lab, EEUU)
    • Michael K. Udvardi (Noble Research Institute, EEUU)
    • Kirankumar S. Mysore (Noble Research Institute, EEUU)
    • Amy Rosenzweig (Northwestern University, EEUU)
    • Daniel Raimunda (Instituto Ferreyra, Argentina)
    • Leonardo Curatti (Instituto Investigaciones en Biodiversidad y Biotecnología, Argentina)
    • Hannetz Roschzttardtz (Pontificia Universidad Católica de Chile, Chile)
    • Camille Larue (ECOLAB, Francia)
    • Hiram Castillo (European Synchrotron Radiation Facility, Francia)
    • Ute Krämer (Ruhr Universität Bochum, Alemania)
    • Daniel Grolimund (Swiss Light Source, Suiza)
    • Hendrik Küpper (Czech Academy of Sciences, Rep. Checa)
    • Louis Grillet (Academia Sinica, Taiwan)
    • Penelope Smith (La Trobe University, Australia)