Metal Homeostasis in the Plant-Microorganism Interaction
Manuel González-Guerrero (IP)
Professor
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigaciones Agrarias, Madrid
Other group members
Research lines
- Transport of metals in legume nodules
- Metal exchange in arbuscular mycorrhizae
- Metal trafficking in biological nitrogen fixation
- Transition metals and innate plant immunity
Representative publications
- González-Guerrero M, Navarro-Gómez C, Rosa-Núñez E, Echávarri-Erasun C, Imperial J, Escudero V (2023). Forging a symbiosis: Transition metal delivery in symbiotic nitrogen fixation. New Phytol. 239: 2113-2125.
- Mihelj P, Abreu I, Moreyra T, González-Guerrero M, Raimunda R (2023). Functional characterization of the Co2+tranporter AitP in Sinorhizobium meliloti: a new player in Fe2+ homeostasis. Appl. Env. Microbiol. 89: e0190122
- Assunçao AGL, Cakmak I, Clemens S, González-Guerrero M, Nawrocki A, Thomine S (2022). Micronutrient homeostasis in plants for more sustainable agriculture and healthier human nutrition. J. Exp. Bot. 73: 1789-1799
- Escudero V, Ferreira- Sánchez D, Abreu I, Sopeña-Torres S, Makarovsky-Saavedra N, Bernal M, Krämer U, Grolimund D, González-Guerrero M, Jordá L (2022). Arabidopsis thaliana Zn2+–efflux ATPases HMA2 and HMA4 are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina BMM. J. Exp. Bot. 73: 339-350.
- Castro-Rodríguez R, Escudero V, Reguera M, Gil-Diez P, Quintana J, Prieto RI, Kumar RK, Brear E, Grillet L, Wen J, Mysore KS, Walker EL, Smith PMC, Imperial J, González-Guerrero M (2021). Medicago truncatula Yellow Stripe-Like7 encodes a peptide transporter required for symbiotic nitrogen fixation. Plant Cell Environ. 44: 1908-1920
- Gavrin A, Loughlin PC, Brear E, Griffith OW, Bedon F, Grotemeyer MS, Escudero V, Reguera M, Qu Y, Mohd-Noor SN, Chen C, Orsorio MB, Rentsch D, González-Guerrero M, Day DA, Smith PMC (2021). Soybean Yellow Stripe-Like7 is a symbiosome membrane peptide essential for nitrogen fixation. Plant Physiol. 186: 581-598
- Senovilla M, Abreu I, Escudero V, Cano C, Bago A, Imperial J, González-Guerrero M (2020) MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots. Mycorrhiza 30: 781-788.
- Castro-Rodríguez R, Abreu I, Reguera M, Novoa-Aponte L, Mijovilovich A, Escudero V, Jiménez-Pastor FJ, Abadía J, Wen J, Mysore KS, Álvarez-Fernández A, Küpper H, Imperial J, González-Guerrero M (2020) Medicago truncatula Yellow Stripe1-Like3 gene is involved in symbiotic nitrogen fixation. J Exp Bot (doi: 10.1093/jxb/eraa390).
- Escudero V, Abreu I, Tejada-Jiménez M, Rosa-Núñez E, Quintana J, Prieto RI, Larue C, Wen J, Villanova J, Mysore KS, Argüello JM, Castillo-Michel H, Imperial J, González-Guerrero M (2020) Medicago truncatula Ferroportin2 mediates iron import into nodule symbiosomes. New Phytol 228: 194-209.
- Escudero V, Abreu I, del Sastre E, Tejada-Jiménez M, Larue C, Novoa-Aponte L, Castillo-González J, Wen J, Mysore KS, Abadía J, Argüello JM, Castillo-Michel H, Álvarez-Fernández A, Imperial J, González-Guerrero M (2020) Nicotianamine synthase2 is required for symbiotic nitrogen fixation in Medicago truncatula Front Plant Sci 10: 1780.
- Ibeas MA, Grant-Grant S, Coronas MF, Vargas JI, Navarro N, Abreu I, Castillo-Michel H, Ávalos-Cembrano N, Paez-Valencia J, Pérez F, González-Guerrero M, Roschzttardtz H (2019) The diverse iron distribution in Eudicotyledoneae seeds: from Arabidopsis to Quinoa. Front Plant Sci 9: 1985 (doi: 3389/fpls.2018.01985).
- Gil-Díez P, Tejada-Jiménez M, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2019) MtMOT1.2 is responsible for molybdate supply to Medicago truncatula Plant Cell Environ 42: 310-320.
- León-Mediavilla J, Senovilla M, Montiel J, Gil-Díez P, Saez A, Kryvoruchko IS, Reguera M, Udvardi MK, Imperial J, González-Guerrero M (2018) MtMTP2-facilitated zinc transport into intracelular compartments is essential for nodule development in Medicago truncatula. Front Plant Sci 9: 990 (doi: 10.3389/fpls.2018.00990).
- Senovilla M, Castro-Rodríguez R, Abreu I, Escudero V, Kryvoruchko I, Udvardi MK, Imperial J, González-Guerrero M (2018) Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation. New Phytol 218: 696-709.
- Kryvoruchko I, Routray P, Senjuti S, Torres-Jerez I, Tejada-Jiménez M, Finney LA, Nakashima J, Pislariu CI, Benedito VA, González-Guerrero M, Roberts DM, Udvardi MK (2018) An iron-activated citrate transporter, MtMATE67, is required for symbiotic nitrogen fixation. Plant Physiol 176: 2315-2329.
- Tejada-Jiménez M, Gil-Díez P, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2017) Medicago truncatula Molybdate Transporter type1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency. New Phytol 216: 1223-1235.
- Abreu I, Sáez A, Castro-Rodríguez R, Escudero V, Rodríguez-Haas B, Senovilla M, Larue C, Grolimund D, Tejada-Jiménez M, Imperial J, González-Guerrero M (2017) Medicago truncatula Zinc-Iron Permease6 provided zinc to rhizobia-infected nodule cells. Plant Cell Environ 40: 2706-2719.
- González-Guerrero M, Escudero V, Saéz A, Tejada-Jiménez M (2016) Transition metal transport in plants and associated endosymbionts: Arbuscular mycorrhizal fungi and rhizobia. Front Plant Sci 29:1088 (doi: 10.3389/fpls.2016.01088).
- Tejada-Jiménez M, Castro-Rodríguez R, Kryvoruchko IS, Lucas MM, Udvardi M, Imperial J, González-Guerrero M (2015) Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia-infected nodule cells. Plant Physiol 168: 258-275.
- Zielazinski EL*, González-Guerrero M*, Subramanian P, Stemmler TL, Argüello JM, Rosenzweig AC (2013) Sinorhizobium meliloti Nia is a P1B-5-ATPase expressed in the nodule during plant symbiosis and is involved in Ni and Fe transport. Metallomics 5: 1614-1623.
- Rodríguez-Haas B, Finney L, Vogt S, González-Melendi P, Imperial J, González-Guerrero M (2013) Iron distribution through the developmental stages of Medicago truncatula Metallomics 5: 1247-1253.
Grants
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González-Guerrero M (IP) (2022-2024). Cytosolic iron trafficking for biological nitrogen fixation. Ministerio de Ciencia e Innovación PID2021-12460OB-100.
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Jordá L, González-Guerrero M (IPs). Death by metals: Unveiling the molecular bases of zinc-mediated immunity. Ministerio de Ciencia en Innovación. TED2021-131769B-100
- Rubio L (PI), González-Guerrero M (2020-2024) BNF-Cereals Phase III. Iron and Mo delivery to pathways to nitrogenase. Bill & Melinda Gates Foundation.
- González-Guerrero M (PI) (2019-2021) Role of plant Cu+-chaperones in intracellular copper trafficking in symbiotic nitrogen fixation. Ministry of Science, Innovation and Universities. PGC2018-095996-B.
- González-Guerrero M (PI) (2016-2018) Diverting metals to Medicago truncatula Ministry of Economy and Competitiveness. AGL-2015-65866-P.
- González-Guerrero M (PI) (2014-2019) Metal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia. European Research Council Starting Grant.
Relevant methods
- Confocal and electronic microscopy
- Visualisation of transition metals in plant tissues
- Purification of membrane proteins
- Metal transport tests on yeasts
Collaborations with other national and international groups
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- Luis M. Rubio (Centro de Biotecnología y Genómica de Plantas, UPM-INIA)
- Lucía Jordá (Centro de Biotecnología y Genómica de Plantas, UPM-INIA)
- Juan Imperial (Instituto de Ciencias Agrarias, CSIC)
- Alberto Bago (Estación Experimental del Zaidín, CSIC)
- Javier Abadía, Ana Álvarez (Estación Experimental de Aula Dei, CSIC)
- José M. Argüello (Worcester Polytechnic Institute, EEUU)
- Luis Vidali (Worcester Polytechnic Institute, EEUU)
- Dennis Dean (Virginia Tech, EEUU)
- Lydia Finney (Argonne National Lab, EEUU)
- Michael K. Udvardi (Noble Research Institute, EEUU)
- Kirankumar S. Mysore (Noble Research Institute, EEUU)
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- Amy Rosenzweig (Northwestern University, EEUU)
- Daniel Raimunda (Instituto Ferreyra, Argentina)
- Leonardo Curatti (Instituto Investigaciones en Biodiversidad y Biotecnología, Argentina)
- Hannetz Roschzttardtz (Pontificia Universidad Católica de Chile, Chile)
- Camille Larue (ECOLAB, Francia)
- Hiram Castillo (European Synchrotron Radiation Facility, Francia)
- Ute Krämer (Ruhr Universität Bochum, Alemania)
- Daniel Grolimund (Swiss Light Source, Suiza)
- Hendrik Küpper (Czech Academy of Sciences, Rep. Checa)
- Louis Grillet (Academia Sinica, Taiwan)
- Penelope Smith (La Trobe University, Australia)
