Plant-Microbe Symbiotic Associations

José Manuel Palacios Alberti (PI)

Professor
Departamento de Biotecnología-Biología Vegetal. Centro de Biotecnología y Genómica de Plantas, CBGP-UPM-INIA, Universidad Politécnica de Madrid, Madrid

0000-0002-2541-8812

jose.palacios@upm.es

PHONE

+34- 910679184 Ext:79184 / 910679139 Ext:79139 ( Lab 251)

Other group members

Luis Rey Navarro

Associate Professor

Marta Albareda Contreras

Assistant Professor

Rocío Álvarez Aragón

Postdoc

Marta Ballesteros Gutiérrez

PhD student

Lucia Domingo Serrano

PhD student

Bruna Fernanda Sousa

PhD student

Lilia Tighilt

PhD student from University of Bejaia, Argelia

Noelia Vicente Santiago

Technician

Research Lines

  • Rhizobium adaptation to host
  • Protein secretion systems
  • Hydrogenase from endosymbiotic bacteria

Representative publications

  • Torres AR, Brito B, Imperial J, Palacios JM, Ciampitti IA, Ruiz-Argüeso T, Hungria M (2020) Hydrogen-uptake genes improve symbiotic efficiency in common beans (Phaseolus vulgaris L.). Antonie van Leeuwenhoek 113: 687–696.
  • Salinero-Lanzarote A, Pacheco-Moreno A, Domingo-Serrano L, Durán D, Ormeño-Orrillo E, Martínez-Romero E, Albareda M, Palacios J, Rey L (2019) The Type VI secretion system of Rhizobium etli Mim1 has a positive effect in symbiosis. FEMS Microbiol Ecol 95: 54 (doi: 10.1093/femsec/fiz054).
  • Albareda M, Pacios LF, Palacios J (2019) Computational analyses, molecular dynamics, and mutagenesis studies of unprocessed form of NiFe hydrogenase reveal the role of disorder for efficient enzyme maturation. BBA-Bioenergetics 1860: 325-340.
  • Durán D, Imperial J, Palacios J, Ruiz-Argüeso T, Göttfert M, Susanne Zehner S, Rey L (2018) Characterization of a novel MIIA domain-containing protein (MdcE) in Bradyrhizobium spp. FEMS Microbiol Lett 365: 276 (doi: 1093/femsle/fnx276).
  • Sánchez-Cañizares C, Jorrín B, Durán D, Nadendla S, Albareda M, Rubio-Sanz L, Lanza M, González-Guerrero M, Prieto RI, Brito B, Giglio MG, Rey L, Ruiz-Argüeso T, Palacios JM, Imperial J (2018) Genomic diversity in the endosymbiotic bacterium Rhizobium leguminosarum. Genes 9: 60 (doi: 3390/genes9020060).
  • Rubio-Sanz L, Brito B, Palacios JM (2018) Analysis of metal tolerance in Rhizobium leguminosarum strains isolated from an ultramafic soil. FEMS Microbiol Lett 365: 10 (doi: 10.1093/femsle/fny010).
  • Msaddak A, Duran D, Rejili M, Mars M, Ruiz-Argüeso T, Imperial J, Palacios J, Rey L (2017) Diverse bacteria affiliated with the genera Microvirga, Phyllobacterium, and Bradyrhizobium nodulate Lupinus micranthus growing in soils of Northern Tunisia. App Env Microbiol 83: e02820-16 (doi: 10.1128/AEM.02820-16).
  • Albareda M, Rodrigue A, Brito B, Ruiz-Argüeso T, Imperial J, Mandrand-Berthelot MA, Palacios JM (2015) Rhizobium leguminosarum HupE is a highly-specific diffusion facilitator for nickel uptake. Metallomics 7: 691-701.
  • Albareda M, Pacios LF, Manyani H, Rey L, Imperial J, Brito B, Ruiz-Argüeso T, Palacios JM (2014) Maturation of Rhizobium leguminosarum hydrogenase in the presence of oxygen requires the interaction of the chaperone HypC and the scaffolding protein HupK. J Biol Chem 289: 21217-21229.
  • Sanchez-Cañizares C, Palacios J (2013) Construction of a marker system for the evaluation of competitiveness for legume nodulation in rhizobium strains. J Microbiol Methods 92: 246-249.

Proyectos

  • Palacios JM (PI) (2019-2021) Mechanisms for adaptation of rhizobia to legume hosts (RhizoAdapt). Ministry of Science, Innovation and Universities. RTI2018-094985.
  • Palacios JM (PI) (2019-2021) Host- and bacterial factors relevant for the interaction of rhizobia with eukaryotic hosts. Programa de Excelencia Severo Ochoa, CBGP-Ministry of Economy, Industry, and Competitiveness. EoI-TSP3-01-CBG.
  • Palacios JM (PI) (2014-2016) Plant-bacteria signalling in the advances stages of the Rhizobium-legume interaction. Ministry of Economy and Competitiveness. BIO2013-43040.
  • Palacios JM (PI) (2011-2013) Metals and metalloenzymes in legume endosymbiotic bacteria: mechanisms for metal incorporation and homeostasis. Ministry of Science and Innovation. BIO2010-15301.
  • Palacios JM (PI) (2007-2010) Biotechnology of hydrogen and oxygen metabolisms for the improvement of legume inoculants. Ministry of Science and Education. BIO2007-64147.

Relevant methods

  • Hydrogen amperometry
  • Proteomic analysis
  • Rhizobium inoculants
  • Plant-microbe interactions under controlled conditions

Collaborations with other national and international research groups

  • Juan Imperial Ródenas (Instituto de Ciencias Agrarias, CSIC, Madrid)
  • Luis Fernandez Pacios (Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Madrid)
  • Javier Alonso Ponga (IGP Lenteja Tierra de Campos, Valladolid)
  • Maria Angela Hungria (EMBRAPA, Brasil)
  • Marie-Andree Mandrand-Berthelot, Agnès Rodrigue (University of Lyon, Francia)
  • Deborah Zamble (University of Toronto, Canadá)
  • Phil Poole (University of Oxford, UK)
  • Michael Gottfert (University of Dresde, Alemania)
  • Erh-Min Lai (Academia Sinica, Taiwan)