• Amaya-Gómez C.V., Flórez-Martínez, D.H., Cayuela M.L., Tortosa G. 2025. Compost and vermicompost improve symbiotic nitrogen fixation, physiology and yield of the Rhizobium-legume symbiosis: A systematic review. Applied Soil Ecology, 210, 106051. DOI: 10.1016/j.apsoil.2025.106051.
• Andrea Jiménez-Leiva, Juan J. Cabrera, María J. Torres, David J. Richardson, Eulogio J. Bedmar, Andrew J. Gates, María J. Delgado*, Socorro Mesa*. 2025. Haem is involved in the NO-mediated regul tion by Bradyrhizobium diazoefficiens NnrR transcription factor. Microbiological research. 297,125181. https://doi.org/10.1016/j.micres.2025.128151.
• Andrea Jiménez-Leiva, Raquel A. Juárez-Martos, Juan J. Cabrera, María J. Torres, Socorro Mesa*, and María J. Delgado*. 2025. Dual oxygen-responsive control by RegSR of nitric oxide reduction in the soybean endosymbiont Bradyrhizobium diazoefficiens. Antioxidants & Redox Signaling. DOI: 10.1089/ars.2024.0710.
• Juan I. Quelas, Juan J. Cabrera, Rocío Díaz-Pena, Lucía Sánchez-Schneider, Andrea Jiménez-Leiva, Germán Tortosa, María J. Delgado, M. Julia Pettinari, Aníbal R. Lodeiro, Coral del Val and Socorro Mesa*. 2024. Pleiotropic effects of PhaR regulator in Bradyrhizobium diazoefficiens microaerobic metabolism. International Journal of Molecular Sciences Int. J. Mol. Sci., 25, 2157. https://doi.org/10.3390/ijms2504215713:1486.
• Alba Hidalgo-García, Germán Tortosa, Pedro J. Pacheco, Andrew J. Gates, David J. Richardson, Eulogio J. Bedmar, Lourdes Girard, María J. Torres & María J. Delgado*.  2023.  Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules.  Journal of Plant Interactions, 18:1, 2251511, DOI: 10.1080/17429145.2023.2251511.
• Pacheco, P.J., Bedmar, E.J., Mesa, S., Tortosa, G., Delgado*, M.J. 2023. Ensifer meliloti denitrification is involved in infection effectiveness and NO emissions by alfalfa root nodules. Plant and Soil, DOI:10.1007/s11104-023-05946-3.
• Parejo S, Cabrera JJ, Jiménez-Leiva A, Tomás-Gallardo L, Bedmar EJ, Gates AJ, Mesa S. (2022). Fine-tuning modulation of oxidation-mediated posttranslational control of Bradyrhizobium diazoefficiens FixK2 transcription factor. Int. J. Mol. Sci. 23:5117. DOI: 10.3390/ijms23095117.
• Pacheco PJ, Cabrera JJ, Jiménez-Leiva A, Bedmar EJ, Mesa S, Tortosa G, Delgado MJ (2022). Effect of Copper on Expression of Functional Genes and Proteins Associated with Bradyrhizobium diazoefficiens Denitrification. International Journal of Molecular Sciences, 23, 3386 (doi: 10.3390/ijms23063386).
• Bueno E, Mania D, Mesa S, Bedmar EJ, Frostegard A, Bakken LR, Delgado MJ (2022). Regulation of the emissions of the greenhouse gas nitrous oxide by the soybean endosymbiont Bradyrhizobium diazoefficiens. International Journal of Molecular Sciences, 2022, 23, 1486.
• Cabrera JJ, Jiménez-Leiva A, Tomás-Gallardo L, Parejo S, Casado S, Torres MJ, Bedmar EJ, Delgado MJ, Mesa S (2021). Dissection of FixK2 protein–DNA interaction unveils new insights into Bradyrhizobium diazoefficiens lifestyles control. Environmental Microbiology, 2021, 23, pp. 6194–6209. (doi: 10.1111/1462-2920.15661).

• Parejo S, Cabrera JJ, Jiménez-Leiva A, Tomás-Gallardo L, Bedmar EJ, Gates AJ, Mesa S. (2022). Fine-tuning modulation of oxidation-mediated posttranslational control of Bradyrhizobium diazoefficiens FixK2 transcription factor. Int. J. Mol. Sci. 23:5117. DOI: 10.3390/ijms23095117.
• Pacheco PJ, Cabrera JJ, Jiménez-Leiva A, Bedmar EJ, Mesa S, Tortosa G, Delgado MJ (2022). Effect of Copper on Expression of Functional Genes and Proteins Associated with Bradyrhizobium diazoefficiens Denitrification. International Journal of Molecular Sciences, 23, 3386 (doi: 10.3390/ijms23063386).
• Cabrera JJ, Jiménez-Leiva A, Tomás-Gallardo L, Parejo S, Casado S, Torres MJ, Bedmar EJ, Delgado MJ, Mesa S (2021). Dissection of FixK2 protein–DNA interaction unveils new insights into Bradyrhizobium diazoefficiens lifestyles control. Environmental Microbiology, 2021, 23, pp. 6194–6209. (doi: 10.1111/1462-2920.15661).
• Bueno E, Mania D, Mesa S, Bedmar EJ, Frostegard A, Bakken LR, Delgado MJ (2022). Regulation of the emissions of the greenhouse gas nitrous oxide by the soybean endosymbiont Bradyrhizobium diazoefficiens. International Journal of Molecular Sciences, 2022, 23, 1486.

• Gas chromatography for detection of greenhouse gases and others
• Nitric oxide (NO) detection through the use of specific electrodes
• Cytochrome c detection c (heme staining)
• Western-blot and protein immunodetection
• Expression and purification of proteins (recombinant and genuine)
• Affinity chromatography, molecular exclusion, ion Exchange
• DNA isolation from environmental samples
• N-cycle genes amplification from environmental samples
• Mass sequencing. Illumina
• Isolation of RNA from free-living cultures and from nodules
• Analysis of gene expression using transcriptional fusions, qRT-PCR and microarrays
• In vitro transcription
• Characterization of promoters using primer extension techniques (RACE, FLOE, primer extension, etc.)
• Protein-DNA interaction: surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and electrophoretic mobility shift assay (EMSA)
• Transcriptomics and proteomics

https://www.eez.csic.es/metabolismo-del-nitrogeno-en-bacterias-rizosfericas